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KMID : 0644120110240030014
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Journal of Oriental Gynecology
2011 Volume.24 No. 3 p.14 ~ p.27
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Effects of Acanthopanacis Cortex Radicis on the Apoptosis in HeLa cell and MCF-7 cell
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Kim Kyung-Sook
Lee Jin-Moo
Lee Chang-Hoon
Jang Jun-Bock
Lee Kyung-Sub
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Abstract
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Objectives:This study was designed to investigate the effects of Acanthopanacis Cortex Radicis extract(ACRE) on the apoptosis in HeLa cell and MCF-7 cell.
Methods:After treatment with various concentration of ACRE, cell growth was evaluated in HeLa cell and MCF-7 cell. Hoechst 33342 staining was performed to estimate DNA fragment effect of ACRE on the apoptosis in HeLa cell and MCF-7 cell. Annexin V/PI apoptosis assay was used to estimate the effects of ACRE on the early apoptosis in HeLa cell and MCF-7 cell. RT-PCR was used to estimate the apoptosis gene expression effect of ACRE on Hela cell MCF-7 cell.
Results:Under 0.1㎎/㎖ of ACRE, cytotoxic effect was not found per NIH3T3 cell. The viability of HeLa cell and MCF-7 cells was significantly decreased ACRE (100㎍/㎖) in HeLa cell and MCF-7 cell, ACRE (50㎍/㎖) in HeLa cell 3 days after treatment, in MCF-7 cell 1&3 days after treatment (p<0.01). DNA fragmentation was observed 3 days after treatment of cl of ACRE on HeLa cell and MCF-7 cell. In Annexin V/PI apoptosis assay, after treatment of 100㎍/㎖ of ACRE, the early apoptotic cell increased both in HeLa cell and MCF-7 cell. In RT-PCR analysis, after treatment of 100㎍/㎖ of ACRE, bcl-2 were decreased and bax, caspase-3 were increased both in HeLa cell and MCF-7 cell.
Conclusions:ACRE appears to have considerable activity on the apoptosis in HeLa cell and MCF-7 cell.
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KEYWORD
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Acanthopanacis Cortex, HeLa cell, MCF-7 cell, Apoptosis, bcl-2
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